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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055809 | PMC |
| http://dx.doi.org/10.1111/pbi.13808 | DOI Listing |
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Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
Maize is one of the major crops that are susceptible to infection and subsequent aflatoxin contamination, which poses a serious health threat to humans and domestic animals. Here, an RNA interference (RNAi) approach called Host-Induced Gene Silencing (HIGS) was employed to suppress the -methyl transferase gene (, also called ), a key gene involved in aflatoxin biosynthesis. An RNAi vector carrying part of the gene was introduced into the B104 maize line.
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State Key Lab for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, Guangxi University, Nanning, China.
Pokkah boeng disease (PBD), a common and highly destructive disease of sugarcane, is mainly caused by Fusarium sacchari. Breeding sugarcane resistant to PBD is challenging due to the limited availability of immune or highly resistant germplasm resources. Host-induced gene silencing (HIGS) based on RNA interference (RNAi) is a promising disease-control method that offers strong disease-targeting ability with low environmental impact.
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Article Synopsis
- Transgenic plants can express double-stranded RNA that silences mRNAs in fungal pathogens, but how this RNA crosses cell membranes during infection is unclear.
- A new protocol allowed researchers to isolate RNA from the leaf surface, revealing a unique pattern distinct from intercellular RNA, suggesting it might be secreted directly rather than through stomata.
- The isolated surface RNAs, primarily from Arabidopsis and including various RNA types, may play a vital role in establishing microbial communities on leaf surfaces.
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