The present study reports a case of hepatic microsporidiosis caused by sp. in brackishwater fish, (Valenciennes, 1837) (n = 60), from the west coast of India. An eight-month study from September 2017 to April 2018 revealed a prevalence of 11.7% for this parasite. The microsporidian showed tissue-specific infection and did not reveal any gross pathology in infected fish. Small whitish cysts containing microspores of size 0.3-0.5 mm were observed in the liver of fish. The range of pyriform microsporidian spore size varied from 2.9-3.77 × 1.85-2.67 µm Scanning electron microscopy of the spores showed a distinct groove on the anterior end of the spore for polar tube extrusion. Polymerase chain reaction (PCR) amplification of the DNA extracted from the microsporidian-infected liver tissue using primers targeting small ribosomal subunit DNA (SSU rDNA) yielded ~ 1340 bp amplicon and the genetic distance analysis showed a 0.2% variation with the reported . Accordingly, in the phylogenetic tree, the present species of clustered with Even though, the morphomeristic characters of the present sp. was marginally different from the reported the SSU rDNA showed considerably higher similarity with Thus, we report the species of as aff. from a new host. This is the first report of a microsporidian from and the first record of the genus from India.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8901909PMC
http://dx.doi.org/10.1007/s12639-021-01419-5DOI Listing

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