AI Article Synopsis
- The calcium-sensing receptor (CaSR), critical for nutrient sensing and physiological processes, was investigated in weaned piglets, focusing on its expression in intestinal cells.
- Primary enterocytes were isolated using specialized techniques and characterized by specific markers to study the presence of CaSR.
- The findings revealed that porcine enterocytes were successfully isolated, but pCaSR was not expressed in the ileal enterocytes or the IPEC-J2 cell line, indicating the need for further exploration of its role.
Article Abstract
The calcium-sensing receptor (CaSR) is a kokumi receptor that plays an essential role in nutrient sensing and animal physiology, growth, and development. Pig CaSR (pCaSR) was identified and characterized in the intestine. However, further research is still needed to confirm the expression of CaSR in the epithelial cells isolated from weaned piglets. In this study, primary enterocytes were isolated and characterized from the ileum of weaned piglets by the Weiser distended intestinal sac technique and fluorescence-activated cell sorting (FACS) based on sucrase-isomaltase (SI) as an enterocyte-specific marker. The expression of CaSR was investigated in both primary enterocytes and the intestinal porcine enterocyte cell line-j2 (IPEC-J2) by droplet digital polymerase chain reaction (ddPCR), immunofluorescence staining, and Western blotting. Results demonstrated that porcine enterocytes could be obtained using FACS with the SI as the enterocyte-specific marker and that pCaSR is not expressed in both porcine ileal enterocytes and IPEC-J2 cells, which specifically identified the expression of pCaSR in ileal enterocytes with sensitive and specific approaches.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9030235 | PMC |
| http://dx.doi.org/10.1093/jas/skac085 | DOI Listing |
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