Spine‑specific downregulation of LAPTM5 expression promotes the progression and spinal metastasis of estrogen receptor‑positive breast cancer by activating glutamine‑dependent mTOR signaling.

Estrogen receptor‑positive (ER) breast cancer (BC) is a malignancy that is prone to metastasis to the spine, which is difficult to treat and often results in poor prognosis. However, the mechanism underlying the tumorigenesis and spinal metastasis of ER BC remains unclear. Lysosomal protein transmembrane 5 (LAPTM5) has been reported as a tumor suppressor in several types of cancer, but its role in ER BC has not been described. Here, by analyzing a gene sequencing dataset and ER BC tissues, tumor‑adjacent normal tissues and spinal metastatic tissues from patients and mouse models, we found that LAPTM5 expression is negatively related to the progression and spinal metastasis of ER BC. Subsequently, experiments demonstrated that downregulation of LAPTM5 expression promoted the proliferation, migration, and chemoresistance of ER BC cells by activating glutamine‑dependent mTOR signaling. A high level of CX3CL1 could inhibit LAPTM5 expression, explaining how ER BC metastasized to the spine. Thus, we found that LAPTM5 functions as a tumor suppressor in ER BC and that the CX3CL/CX3CR1/LAPTM5/glutamine axis mediates the spinal metastasis of ER BC. This axis may be a promising therapeutic target for ER BC.