Anti-Inflammatory and Anti-Remodelling Potential of Ethanol Extract in Combination of Asthma and Coal Dust Models.

Background: Combination of asthma and coal dust is a chronic and recurring airway disease related to inflammation cell activation. The flowering plants native to South Kalimantan exhibit a broad therapeutic potential, like anti-inflammatory and anti-remodelling properties. This study aims to analyze the effect of ethanol extract of leaves (EERTL) nebulizer on the number of inflammatory cells and histomorphometry of lung tissue in a mice-like model of a combination of asthma and coal dust.

Methods: The 24 BALB/c mice were divided into four treatment groups (n= 6 per group), were sensitized with normal saline (K), OVA + coal dust (P1), OVA + coal dust + salbutamol (P2), and OVA + coal dust + EERTL (P3). Eosinophil cells, neutrophils, lymphocytes, epithelial thickness, smooth muscle, fibrosis subepithelial bronchioles, and the number of goblet cells as indicators of anti-inflammatory and anti-remodelling airways.

Results: The number of eosinophils, neutrophils, and lymphocytes cells are given salbutamol or EERTL was significantly lower than the OVA-sensitized and coal dust exposure group only. There are meaningful differences in the average thickness of the epithelium, smooth muscle, and subepithelial fibrosis of bronchiolus. The histopathology picture of goblet cells showed an increase in the number and size (hyperplasia) in OVA-sensitized and coal dust exposure compared to another group.

Conclusion: It was concluded that the EERTL nebulizer could reduce inflammatory cells and remodelling process from bronchoalveolar lavage in the mice combination of asthma and coal dust models.