Electrochemical aptasensing strategy based on a multivariate polymertitanium-metal-organic framework for zearalenone analysis.

An electrochemical aptasensing strategy was developed with a novel bioplatform based on a multivariate titanium metal-organic framework, i.e. MTV polyMOF(Ti), to detect zearalenone (ZEN). MTV polyMOF(Ti) was prepared by using mixed linkers of polyether polymer (pbdc-xa or L, pbdc = poly(1,4-benzenedicarboxylate) and 1,4-benzenedicarboxylic acid (Hbdc or L) as well as tetrabutyl titanate as nodes (MTV polyMOF(Ti)-L). Compared with Ti-MOFs synthesized by using the single ligand of L or L, MTV polyMOF(Ti)-L shows more porous structure assembled with multilayered nanosheets. In light of the improved electrochemical activity and strong bioaffinity to the aptamer, the aptasensor based on MTV polyMOF(Ti)-L shows excellent performance for detecting ZEN with the ultralow detection limit at fg mL level in the linear range of 10 fg mL to 10 ng mL, along with good selectivity, reproducibility, stability, regenerability, and applicability.