AI Article Synopsis
- Osteoarthritis (OA) is a prevalent condition in older adults, but the molecular mechanisms behind its development and progression are still unclear; this study explored these mechanisms using a competing endogenous RNA (ceRNA) network approach.
- Researchers analyzed various mRNA, miRNA, and lncRNA datasets to identify differentially expressed genes (DEGs) and constructed a ceRNA network to explore their biological functions through enrichment analyses, as well as assessing immune cell presence in OA versus healthy samples.
- Key findings revealed significant players like hsa-mir-425-3p and dual specificity phosphatase 1 in the ceRNA network, highlighting their roles in important biological processes linked to immune responses and OA progression, suggesting potential targets for further
Article Abstract
Background: Osteoarthritis (OA) is one of the most common diseases in elderly people; however, the correlation between molecular alterations and the occurrence and progression of OA are still not well understood. We conducted this study to investigate the molecular changes in OA via the competing endogenous ribonucleic acid (ceRNA) network.
Methods: We downloaded the messenger RNA (mRNA) data set, GSE48556, the microRNA (miRNA) data set, GSE105027, and the long non-coding (lncRNA) data set, GSE126963 from the Gene Expression Omnibus (GEO) database, and examined the differentially expressed genes (DEGs) in these data sets. Further, we constructed a ceRNA network of the differentially expressed miRNAs, mRNAs, and lncRNAs. To determine the biological functions of the ceRNA network, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses. Finally, we conducted an immune cell infiltration analysisusing single-sample gene set enrichment analysis to examine the abundance of immune cells in healthy and OA patients, and compared the infiltration of 28 immune cells between the healthy and OA samples. We also analyzed the relationship between the abundance of immune cells and mRNA expression levels in the ceRNA network.
Results: Ultimately hsa-mir-425-3p, dual specificity phosphatase 1, and 24 lncRNAs were identified in the ceRNA network. The functional enrichment analyses showed that these lncRNAs, miRNAs, and mRNAs are involved in various significant biological process, such as the regulation of leukocyte migration, Mitogen-Activated Protein (MAP) kinase tyrosine/serine/threonine phosphatase activity, the interleukin-17 signaling pathway, the tumor necrosis factor signaling pathway, and osteoclast differentiation, and can also have a strong effect on immune cell infiltration.
Conclusions: The dual-specificity phosphatase 1-specific ceRNA network can be used as a diagnostic tool to assess the progression of OA patients.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904995 | PMC |
| http://dx.doi.org/10.21037/atm-21-6711 | DOI Listing |
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