The saccharification of cellulose and hemicellulose is essential for utilizing lignocellulosic biomass as a biofuel. While cellulose is composed of glucose only, hemicelluloses are composed of diverse sugars such as xylose, arabinose, glucose, and galactose. is a good potential candidate for biofuel production using hemicellulose as this archaeon simultaneously utilizes various sugars. However, has to be manipulated because the enzyme that breaks down hemicellulose is not present in this species. Here, we engineered to utilize xylan as a carbon source by introducing xylanase and β-xylosidase. Heterologous expression of β-xylosidase enhanced the organism's degradability and utilization of xylooligosaccharides (XOS), but the mutant still failed to grow when xylan was provided as a carbon source. exhibited the ability to degrade xylan into XOS when xylanase was introduced, but no further degradation proceeded after this sole reaction. Following cell growth and enzyme reaction, successfully utilized xylan in the synergy between xylanase and β-xylosidase.
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| http://dx.doi.org/10.4014/jmb.2202.02016 | DOI Listing |
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