AI Article Synopsis
- - The study investigated the effects of re-extending frozen-thawed donkey semen in two different mediums—seminal plasma (SP) and INRA96—on semen quality post-thaw and pregnancy rates in jennies.
- - Semen was collected from two fertile Amiata donkey stallions, frozen with specific preservatives, and seminal plasma was stored for later use.
- - Results showed higher pregnancy rates when jennies were inseminated with sperm re-extended in SP, indicating potential benefits of using autologous seminal plasma for improving success rates in artificial insemination.
Article Abstract
In the literature, the very low pregnancy rates after artificial insemination (AI) with frozen semen in donkeys were improved in one study after re-extension of the frozen-thawed semen in autologous seminal plasma. The aims of our study were (1) to describe in vitro post-thaw parameters of donkey jackass semen after re-extension in seminal plasma (SP) or in INRA96 and (2) to compare pregnancy rates in jennies bred with frozen-thawed semen using two different AI protocols. Semen collected from two Amiata donkey stallions, known to be fertile, was frozen in INRA96 supplemented with 2% egg yolk, and 2.2% glycerol. From the same jacks, seminal plasma was filtered, and stored at -20°C. According to the in vitro analysis, the post-thaw re-extension in SP some kinematic parameters decreased more over time. Two different AI protocols were tested: inseminating deeply into the uterine horn (DHAI) with or without thawed semen re-extension in SP. Higher pregnancy rates were obtained with re-extension in SP than when AIs were performed without SP. Further studies are needed to confirm the advantage of re-extension in autologous SP of donkey frozen-thawed semen before AI in jennies.
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| http://dx.doi.org/10.1016/j.jevs.2022.103932 | DOI Listing |
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