Infects Glial Cells and Invades the Central Nervous System the Olfactory and Trigeminal Nerves.

causes neonatal meningitis and can also infect the adult central nervous system (CNS). can cross the blood-brain barrier but may also reach the CNS other paths. Several species of bacteria can directly invade the CNS the olfactory and trigeminal nerves, which extend between the nasal cavity and brain and injury to the nasal epithelium can increase the risk/severity of infection. Preterm birth is associated with increased risk of infection and with nasogastric tube feeding. The tubes, also used in adults, can cause nasal injuries and may be contaminated with bacteria, including . We here investigated whether could invade the CNS after intranasal inoculation in mice. rapidly infected the olfactory nerve and brain. Methimazole-mediated model of nasal epithelial injury led to increased bacterial load in these tissues, as well as trigeminal nerve infection. infected and survived intracellularly in cultured olfactory/trigeminal nerve- and brain-derived glia, resulting in cytokine production, with some differences between glial types. Furthermore, a non-capsulated was used to understand the role of capsule on glial cells interaction. Interestingly, we found that the capsule significantly altered cytokine and chemokine responses and affected intracellular survival in trigeminal glia. In summary, this study shows that can infect the CNS the nose-to-brain path with increased load after epithelial injury, and that the bacteria can survive in glia.