Effect of Hcp Iron Ion Regulation on the Interaction Between With Human Pulmonary Alveolar Epithelial Cells and Biofilm Formation.

is a type of bacterial nosocomial infection with severe drug resistance. Hemolysin co-regulated protein (Hcp) is a marker of activated type VI secretion system (T6SS), a key secretory system that promotes Gram-negative bacteria colonization, adhesion, and invasion of host cells. Hcp is also regulated by iron ions (Fe). In this study, an ATCC17978 deletion strain (ATCC17978Δ), an complement strain (ATCC17978Δ ), and an -green fluorescent protein (GFP) strain were constructed and used to investigate the role of in bacterial adhesion to cells (human pulmonary alveolar epithelial cells (HPAEpiC)) and biofilm formation. Our results indicate that the inhibitory concentrations of the three strains (ATCC17978 wild type, ATCC17978Δ, and ATCC17978Δ) were drug-sensitive strains. gene and iron ions might be involved in promoting the formation of a biofilm and host-bacteria interaction. Iron ions affected the ability of to adhere to cells, as there was no significant difference in the bacterial numbers when assessing the adhesion of the three strains to HPAEpiC in the presence of iron ion concentrations of 0 μM ( = 3.1800, = 0.1144), 25 μM ( = 2.067, = 0.2075), 100 μM ( = 30.52, = 0.0007), and 400 μM ( = 17.57, = 0.0031). The three strains showed significant differences in their ability to adhere to HPAEpiC. The numbers of bacteria adhesion to HPAEpiC were ATCC17978Δ>ATCC17978Δ>ATCC17978 in descending order. gene was positively regulated by iron ions in the bacteria-cells' co-culture. It is speculated that the effect of iron ions on the interaction between and HPAEpiC might be related to the transport function of and bacterial immune escape mechanisms.