Activating PGC-1α-mediated signaling cascades in the aorta contributes to the amelioration of vascular senescence and atherosclerosis by 2,3,4',5-tetrahydroxystilbene-2-O-β-d-glycoside.

Background: 2,3,4',5-tetrahydroxystilbene-2-O-β-d-glycoside (TSG), the main active polyphenolic component of Polygonum multiflorum, possesses many pharmacological activities. Its anti-aging effect influences a variety of tissues with diverse mechanisms. However, the effectiveness and exact mechanisms of TSG against vascular senescence in atherosclerosis remain unclear. The present study is aimed to investigate the effects of TSG against vascular senescence in atherosclerosis both in vivo and in vitro, and the possible underlying mechanisms focusing on aortic peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α)-mediated signaling cascades which have never been studied.

Methods: In vivo, 12-mo-old male LDLr mice were randomly separated into control, high-fat diet (HFD), and TSG -treatment groups. At the end of the 12 weeks, the blood samples and aorta tissues of mice were collected for further analysis. In vitro, to mimic the condition of endothelial senescence in hyperlipidemic mice, human aortic endothelial cells (HAECs) were incubated with oxidized low-density lipoprotein (ox-LDL) to induce senescence.

Results: TSG administration improved lipid profiles, ameliorated HFD-exacerbated vascular senescence and atherosclerosis. The protective effect of TSG via inhibiting telomere malfunction, oxidative stress, and mitochondrial damage was found both in vivo and in vitro. Notably, TSG administration increased aortic PGC-1α mRNA and protein expression along with the regulation of its targeted genes TERT, NRF1, TFAM, Mn-SOD, and catalase. Further, by using PGC-1α siRNA in ox-LDL-treated HAECs, it is proved that TSG reduced endothelial senescence, telomere malfunction, oxidative stress, and mitochondrial damage at least partly through activating the PGC-1α pathway.

Conclusions: These results provide new evidence for TSG in the treatment of atherosclerosis and the activation of aortic PGC-1α is involved in its beneficial effects.