AI Article Synopsis
- * Ions over one megadalton can be trapped for seconds while traveling long distances, enabling accurate mass analysis with high resolution (over 100,000).
- * We found that high-mass ions can lose solvent molecules and, occasionally, an elementary charge, but we can correct for these changes, leading to significant improvements in ion sampling and mass resolution.
Article Abstract
To enhance the performance of charge-detection mass spectrometry, we investigated the behaviour of macromolecular single ions on their paths towards and within the Orbitrap analyser. Ions with a mass beyond one megadalton reach a plateau of stability and can be successfully trapped for seconds, travelling a path length of multiple kilometres, thereby enabling precise mass analysis with an effective resolution of greater than 100,000 at a mass-to-charge ratio of 35,000. Through monitoring the frequency of individual ions, we show that these high-mass ions, rather than being lost from the trap, can gradually lose residual solvent molecules and, in rare cases, a single elementary charge. We also demonstrate that the frequency drift of single ions due to desolvation and charge stripping can be corrected, which improves the effective ion sampling 23-fold and gives a twofold improvement in mass precision and resolution.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068510 | PMC |
| http://dx.doi.org/10.1038/s41557-022-00897-1 | DOI Listing |
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