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Live cell tracking of macrophage efferocytosis during embryo development in vivo. | LitMetric

AI Article Synopsis

  • Researchers created a fluorescent reporter called CharON to track dying cells (apoptotic cells) and their removal by immune cells (efferocytosis) in living organisms.
  • Using CharON, they discovered differences in how macrophages (a type of immune cell) handle the cleanup of high numbers of dying cells during embryo development.
  • Some macrophages took on too many dead cells, which hindered their ability to clear away other debris from wounds, highlighting new insights into cell death and immune responses.

Article Abstract

Apoptosis of cells and their subsequent removal through efferocytosis occurs in nearly all tissues during development, homeostasis, and disease. However, it has been difficult to track cell death and subsequent corpse removal in vivo. We developed a genetically encoded fluorescent reporter, CharON (Caspase and pH Activated Reporter, Fluorescence ON), that could track emerging apoptotic cells and their efferocytic clearance by phagocytes. Using expressing CharON, we uncovered multiple qualitative and quantitative features of coordinated clearance of apoptotic corpses during embryonic development. When confronted with high rates of emerging apoptotic corpses, the macrophages displayed heterogeneity in engulfment behaviors, leading to some efferocytic macrophages carrying high corpse burden. Overburdened macrophages were compromised in clearing wound debris. These findings reveal known and unexpected features of apoptosis and macrophage efferocytosis in vivo.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7612538PMC
http://dx.doi.org/10.1126/science.abl4430DOI Listing

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