The presence of antimicrobial-resistant pathogens such as strains in the food supply is dangerous. The aim of this study was to assess the prevalence of strains in Greek meat products and evaluate their phenotypes and genotypes. One hundred and ten meat specimens were cultured for the isolation of . In positive specimens, PCR (Polymerase Chain Reaction) analysis was performed to confirm the presence of . Genotypic and phenotypic evaluation of the isolated strains included multiplex immunoassay for the detection of carbapenemases, and PCR screening for the detection of resistance and virulence genes. strains were recovered in 90 (81.8%) meat samples. The gene was identified in 30 (33.3%) isolates, while the and genes were present in 15 (16.7%) and 65 (72.2%) isolates, respectively. Sixty-five isolates (72.2%) were found to carry at least one resistance gene; of these, the -like was the most commonly identified gene in 40 (61.5%) isolates, followed by the -48 like gene in 20 isolates (30.8%). A high frequency of foodborne in Greece was found. Our results indicate that most strains carried resistance and virulence genes, indicating a high pathogenic potential and a significant risk to human health.
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| http://dx.doi.org/10.3390/foods11050708 | DOI Listing |
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