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A cross-species spatiotemporal proteomic analysis identifies UBE3A-dependent signaling pathways and targets. | LitMetric

AI Article Synopsis

  • * Research using proteomics on AS mouse and rat models revealed significant disruptions in proteasomal pathways and synaptic proteins throughout brain development, especially in adulthood.
  • * The study identified TKT as a key UBE3A substrate and provided a detailed overview of protein changes in AS, emphasizing the importance of UBE3A restoration for future therapeutic approaches, along with a multi-species proteomic resource for AS research.

Article Abstract

Angelman syndrome (AS) is a severe neurodevelopmental disorder caused by the loss of neuronal E3 ligase UBE3A. Restoring UBE3A levels is a potential disease-modifying therapy for AS and has recently entered clinical trials. There is paucity of data regarding the molecular changes downstream of UBE3A hampering elucidation of disease therapeutics and biomarkers. Notably, UBE3A plays an important role in the nucleus but its targets have yet to be elucidated. Using proteomics, we assessed changes during postnatal cortical development in an AS mouse model. Pathway analysis revealed dysregulation of proteasomal and tRNA synthetase pathways at all postnatal brain developmental stages, while synaptic proteins were altered in adults. We confirmed pathway alterations in an adult AS rat model across multiple brain regions and highlighted region-specific differences. UBE3A reinstatement in AS model mice resulted in near complete and partial rescue of the proteome alterations in adolescence and adults, respectively, supporting the notion that restoration of UBE3A expression provides a promising therapeutic option. We show that the nuclear enriched transketolase (TKT), one of the most abundantly altered proteins, is a novel direct UBE3A substrate and is elevated in the neuronal nucleus of rat brains and human iPSC-derived neurons. Taken together, our study provides a comprehensive map of UBE3A-driven proteome remodeling in AS across development and species, and corroborates an early UBE3A reinstatement as a viable therapeutic option. To support future disease and biomarker research, we present an accessible large-scale multi-species proteomic resource for the AS community ( https://www.angelman-proteome-project.org/ ).

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135630PMC
http://dx.doi.org/10.1038/s41380-022-01484-zDOI Listing

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