Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing.

J Virol Methods

KU Leuven, Rega Institute, Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, 3000 Leuven, Belgium. Electronic address:

Published: June 2022

SARS-CoV-2 has kept the world in suspense for almost 2 years now. The virus spread rapidly worldwide and several variants of concern have emerged: Alpha, Beta, Gamma, Delta and recently Omicron. A rapid method to detect key mutations is needed because these variants may jeopardize the effectiveness of immune protection following vaccination or past infection. This article describes an easy, cheap and fast method for the detection of mutations in the spike protein that are indicative for specific variants. This method can easily distinguish Omicron from other variants.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896866PMC
http://dx.doi.org/10.1016/j.jviromet.2022.114512DOI Listing

Publication Analysis

Top Keywords

variants
5
fast detection
4
detection sars-cov-2
4
sars-cov-2 variants
4
variants including
4
including omicron
4
omicron one-step
4
one-step rt-pcr
4
rt-pcr sanger
4
sanger sequencing
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!