Sex Influences Age-Related Changes in Natural Antibodies and CD5 B-1 Cells.

Natural Abs are primarily produced by B-1 cells and are essential for protection against The incidence and mortality rate for pneumococcal infection increases dramatically after age 65, disproportionately affecting males in both human and murine systems. To date, there is a significant gap in our understanding of the relationship among sex, aging, natural IgM efficacy, and the natural IgM repertoire. Our investigation demonstrates that the protective capacity of serum IgM against pneumococcal infection is maintained in IgM obtained from aged female mice but absent in IgM from aged male mice. To understand this difference in protective capacity, we examined serum Ig, discovering that the protective change was not associated with shifts in levels of phosphorylcholine (PC)- or pneumococcal capsular polysaccharide serotype 3-specific IgM. Interestingly, we observed that aged females have an increase in the total number of CD5 B-1 cells, higher serum IL-5 levels, and a larger percentage of aged female CD5 B-1 cells that express CD86 as compared with aged males. Furthermore, single-cell IgM repertoire analysis from peritoneal PC, splenic PC, and bone marrow CD5 B-1 cell subsets demonstrated greater diversity with age and a higher level of germline status in female mice than previously observed in studies of aged male mice. Aged female CD5 B-1 cells also expressed higher levels of transcripts associated with cell activity and self-renewal, such as and Taken together, these data indicate that females maintain a more diverse and active CD5 B-1 cell pool and natural IgM repertoire, which has implications for sex-related susceptibility to infection and disease.