First Report of White Rust on Sweetpotato Caused by in Guangdong, China.

Plant Dis

Guangdong Academy of Agricultural Sciences, 117866, Guangzhou, Guangdong, China;

Published: March 2022

AI Article Synopsis

  • In October 2021, white rust disease was discovered affecting sweetpotato leaves in Yunfu, Guangdong, resulting in visible symptoms such as yellowing, browning, and the formation of powdery pustules on the leaves.
  • Laboratory analysis using scanning electron microscopy and PCR techniques identified the pathogen responsible for the disease, suggesting it belongs to the genus that affects sweetpotato.

Article Abstract

Sweetpotato () is the eighth major food crop cultivated worldwide with annual production of 89.5 million tons (FAO 2020). China is the world's biggest producer of sweetpotato, and Guangdong Province has the fourth-largest sweetpotato growing area and the biggest sweetpotato market in China (Huang et al. 2020a). Sweetpotato leaves are a key organ providing nutrients for humans and animals, and are popular with customers in Guangdong. On October 14, 2021, a white rust affecting sweetpotato leaves was observed in the fields of Yunfu, Guangdong (22°54'55''N, 112°02'40''E) when conditions were humid, rainy and relatively mild. The adaxial surface of the infected leaves initially exhibited irregular light-yellow or yellow spots, which gradually turned to brown and necrotic. Meanwhile, tiny, powdery, chalky-white pustules, typical of white rust, dispersed individually or in clusters were observed on the corresponding underside of lesions, resulting in wrinkled leaves or abscission. For further analysis in the laboratory, affected leaf pieces (5 mm × 5 mm) containing raised pustules were examined using a scanning electron microscope (S-3400N-Ⅱ, Hitachi, Japan) at 5kv. The micrographs revealed numerous cylindrical-shaped sporangia released from broken pustules. The surfaces of globose oospores were covered with tiny papillae in a reticular pattern. Based on the morphological analyses, the pathogen was preliminarily identified as an sp. Crude genomic DNA of a few pustules from the diseased leaves were extracted and subjected to PCR amplification using a 2×T5 Direct PCR kit (TSE011, Tsingke, China) with the primers, ITS1/ITS4 (White et al. 1990). PCR products were detected using agarose gel electrophoresis and sequenced by Tsingke company (Guangzhou, China). The sequences were compared against the NCBI database using the BLASTn search tool. The two best-matched alignments with over 90% query coverage showed that ITS sequence amplified from the sample, which was deposited in GenBank (OM182104), was ≥97% identical to those from two isolates of from China (AY742741) and Korea (DQ643920). primarily causes white rust on sweetpotato (Moyer and Clark 2013) and is an obligate parasite not culturable in vitro. To demonstrate pathogenicity, spores collected from symptomatic sweetpotato leaves were mixed with sterile water and sprayed onto leaves of three healthy sweetpotato plants. Inoculated plants were then placed at 21℃ and 93% relative humidity. Three other healthy plants treated with sterile water without spores served as the control group. After 12 days, chlorosis and necrosis were observed on the upper leaf surface; and raised white pustules appeared on the lower leaf surface. No symptoms were observed in the noninoculated control plants. To the best of our knowledge, this is the first report of white rust, caused by , affecting sweetpotato in Guangdong, China. White rust on sweetpotato has also been reported in other provinces in China (Huang et al. 2020b), and the detrimental effects and control measures of this disease should be studied further.

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-01-22-0031-PDNDOI Listing

Publication Analysis

Top Keywords

white rust
24
rust sweetpotato
16
sweetpotato
12
sweetpotato leaves
12
report white
8
china
8
guangdong china
8
sweetpotato guangdong
8
china huang
8
sterile water
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!