AI Article Synopsis
- Accurate immune cell analysis requires a large quantity of Peripheral Blood Mononuclear Cells (PBMCs) from blood samples, but traditional methods often produce low yields and contamination.
- An optimized protocol using PBS with EDTA has been developed to enhance PBMC yield, particularly from pregnant patients, allowing for better analysis of immune cell types like CD4+, CD8+, and Regulatory T Cells.
- This refined technique may be applied to various immune cell populations, with detailed instructions available at the SepMate website.
Article Abstract
To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMate isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cells. Here, we describe an optimized protocol, using PBS with EDTA to increase PBMC yield from pregnant patients. This protocol enables analysis of CD4+, CD8+, and Regulatory T Cells and is potentially applicable to any immune cell population. For complete details on the use and execution of this protocol, please refer to the SepMateTM website https://www.stemcell.com/products/brands/SepMateTM-pbmc-isolation.html.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888982 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101204 | DOI Listing |
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