Identification of Mutants With Increased Susceptibility to .

Clubroot, caused by the obligate parasite , is one of the most devastating diseases of canola () in Canada. The identification of novel genes that contribute to clubroot resistance is important for the sustainable management of clubroot, as these genes may be used in the development of resistant canola cultivars. Phospholipase As (PLAs) play important roles in plant defense signaling and stress tolerance, and thus are attractive targets for crop breeding. However, since canola is an allopolyploid and has multiple copies of each gene, it is time-consuming to test the functions of directly in this crop. In contrast, the model plant has a simpler genetic background and only one copy of each . Therefore, it would be reasonable and faster to validate the potential utility of genes in first. In this study, we identified seven homozygous knockout/knockdown mutants of , and tested their performance following inoculation with . Four mutants (α, γ, , β, δ) developed more severe clubroot than the wild-type, suggesting increased susceptibility to . The homologs of these () in () were identified through Blast searches and phylogenic analysis. Expression of the was subsequently examined in transcriptomic datasets generated from canola infected by , and promising candidates for further characterization identified.