Objectives: This study aimed to produce a recombinant protein vaccine candidate based on an epitope of spike protein from Indonesian SARS-CoV-2 to provide immunogenicity and protection against future infection.
Methods: A reverse vaccinology approach was used to identify potential vaccine candidates by screening the pathogen's genome through computational analyses.
Results: Epitope vaccine candidates with the amino acid sequence of FKNHTSPDV were selected. This peptide is hydrophilic, does not induce autoimmune and allergic reactions, is antigenic, is classified as a stable protein, and is predicted to be present in the cell membrane. The selected epitope sequences were inserted into the plasmid vector pcDNA3.1(+) N-GST (thrombin). Inclusion of additional features of the gene encoding glutathione-S transferase, which can increase antigen expression and solubility, and the genes encoding NSP 1-4 proteins, which are essential in replication, added value to the produced recombinant protein.
Conclusion: Recombinant protein vaccine candidates with the FKNHTSPDV epitope have parameters sufficient for production on a laboratory scale for further testing.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881762 | PMC |
| http://dx.doi.org/10.1016/j.jtumed.2022.02.007 | DOI Listing |
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