AI Article Synopsis
- - Meiotic recombination is crucial for genetic diversity in gametes, but it's not thoroughly studied in livestock like cattle, despite being more understood in humans and some model organisms.
- - Researchers sequenced 143 individual sperm from two Holstein bulls to analyze high-resolution meiotic recombination events, finding that these events are more frequent at the ends of chromosomes and are a natural part of meiosis.
- - This study represents the first extensive whole-genome sequencing of single sperm in livestock, offering valuable insights for future research on recombination, genome stability, and male infertility.
Article Abstract
Background: Meiotic recombination is one of the important phenomena contributing to gamete genome diversity. However, except for human and a few model organisms, it is not well studied in livestock, including cattle.
Results: To investigate their distributions in the cattle sperm genome, we sequenced 143 single sperms from two Holstein bulls. We mapped meiotic recombination events at high resolution based on phased heterozygous single nucleotide polymorphism (SNP). In the absence of evolutionary selection pressure in fertilization and survival, recombination events in sperm are enriched near distal chromosomal ends, revealing that such a pattern is intrinsic to the molecular mechanism of meiosis. Furthermore, we further validated these findings in single sperms with results derived from sequencing its family trio of diploid genomes and our previous studies of recombination in cattle.
Conclusions: To our knowledge, this is the first large-scale single sperm whole-genome sequencing effort in livestock, which provided useful information for future studies of recombination, genome instability, and male infertility.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8898482 | PMC |
| http://dx.doi.org/10.1186/s12864-022-08415-w | DOI Listing |
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