AI Article Synopsis
- Several monoclonal antibodies have been approved to treat COVID-19, but their development is often slow due to the need to test many candidates.
- A new method combines target-ligand blocking and a B cell receptor-sequencing approach called LIBRA-seq, which helps quickly identify effective neutralizing antibodies.
- This innovative combination could significantly speed up and enhance the discovery process for new antibodies that fight against SARS-CoV-2.
Article Abstract
Although several monoclonal antibodies (mAbs) targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been approved for coronavirus disease 2019 (COVID-19) therapy, development was generally inefficient, with lead generation often requiring the production and testing of numerous antibody candidates. Here, we report that the integration of target-ligand blocking with a previously described B cell receptor-sequencing approach (linking B cell receptor to antigen specificity through sequencing (LIBRA-seq)) enables the rapid and efficient identification of multiple neutralizing mAbs that prevent the binding of SARS-CoV-2 spike (S) protein to angiotensin-converting enzyme 2 (ACE2). The combination of target-ligand blocking and high-throughput antibody sequencing promises to increase the throughput of programs aimed at discovering new neutralizing antibodies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378442 | PMC |
| http://dx.doi.org/10.1038/s41587-022-01232-2 | DOI Listing |
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