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The Impact of a Very-Low-Calorie Ketogenic Diet on Monocyte Subsets of Patients with Obesity: A Pilot Study.
Nutrients
January 2025
Department of Experimental Medicine, Section of Medical Pathophysiology, Food Science and Endocrinology, Sapienza University of Rome, 00161 Rome, Italy.
Obesity is closely linked to chronic low-grade inflammation and the development of cardio-metabolic comorbidities. Monocyte subsets, which are crucial in immune responses, have been reported to be altered in individuals with obesity, potentially exacerbating inflammation. Although very-low-calorie ketogenic diets (VLCKDs) are recognized for their efficacy in promoting weight loss and improving metabolic health, their impact on circulating monocyte subsets remains poorly understood.
View Article and Find Full Text PDFAssessment of Antibiotic Resistance Among Isolates of spp. and spp. in Wildlife and Their Environment from Portugal: A Positive Epidemiologic Outcome.
Pathogens
January 2025
MicroART-Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal.
One of the significant challenges facing modern medicine is the rising rate of antibiotic resistance, which impacts public health, animal health, and environmental preservation. Evaluating antibiotic resistance in wildlife and their environments is crucial, as it offers essential insights into the dynamics of resistance patterns and promotes strategies for monitoring, prevention, and intervention. and genera isolates were recovered from fecal samples of wild animals and environmental samples using media without antibiotic supplementation.
View Article and Find Full Text PDFOptimization and Validation of an Ultra-Performance Liquid Chromatography with Quadrupole Detector Mass Spectrometry Quantification Method for the Simultaneous Detection of Tazarotene and Tazarotenic Acid in Porcine Skin: An In Vitro Study.
Int J Mol Sci
January 2025
Faculty of Pharmaceutical Sciences, University of Iceland, 107 Reykjavik, Iceland.
Exploring tazarotene, a third-generation retinoid for potential hand osteoarthritis treatment, this study presents the development and validation of an ultra-performance liquid chromatography with quadrupole detector mass spectrometry (UPLC-QDa) method for the simultaneous quantification of tazarotene and tazarotenic acid, its active metabolite, in porcine skin. Method development involved a design-of-experiments approach for chromatographic optimization of gradient steepness, organic solvent volume, column temperature, capillary voltage, flow rate, and cone voltage. Central composite orthogonal design was used to optimize peak area, peak width, retention time, and resolution.
View Article and Find Full Text PDFConcordant Patterns of Population Genetic Structure in Food-Deceptive Orchids.
Genes (Basel)
January 2025
Faculty of Biology, University of Bialystok, Ciołkowskiego 1J Street, 15-245 Białystok, Poland.
Background: The patterns of inbreeding coefficients () and fine spatial genetic structure (FSGS) were evaluated regarding the mating system and inbreeding depression of food-deceptive orchids, , var. , and , from NE Poland.
Methods: We used 455 individuals, representing nine populations of three taxa and AFLPs, to estimate percent polymorphic loci and Nei's gene diversity, which are calculated using the Bayesian method; ; ; FSGS with the pairwise kinship coefficient (); and AMOVA in populations.
The association of activity patterns on female reproductive diseases: a prospective cohort study of UK biobank.
BMC Public Health
January 2025
Department of Obstetrics and Gynecology, the First Affiliated Hospital of Harbin Medical University, 23 Postal Street, Harbin, 150081, Heilongjiang, P. R. China.
Objective: Little is known about the role of timing of physical activity in female reproductive disorders. These disorders include polycystic ovary syndrome (PCOS), heavy menstrual bleeding (HMB), endometriosis, infertility, and pregnancy-related disorders. This study aims to investigate the associations of activity patterns with female reproductive diseases.
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