Investigations on lithographically formed cavities of surface-imprinted polymers (SIP) can help to gain deeper understanding on cell recognition with SIPs: it is known that surface topography and biomolecules transferred during surface imprinting contribute to cell adhesion. In this work, SIPs synthesized two different imprinting techniques, namely stamp imprinting and polymerization of Pickering emulsions, were investigated and compared to each other, using atomic force microscopy (AFM) and Peak Force Quantitative Nano Mechanics (PF-QNM). We focused on SIPs based on poly(styrene--divinylbenzene) as model polymer and as model template for cell imprinting. Both imprinting approaches led to cavities that revealed nanostructures within the imprints. Stamp imprinting cavities feature low surface roughness and channel structures that resemble the negative pattern of the bacteria on the stamp and their filaments, while SIPs synthesized polymerization of Pickering emulsions reveal globular nanostructures accumulating in the imprints. AFM phase imaging and adhesion mapping using PF-QNM show that these globular structures are remainders of the imprinted cells, most likely lipopolysaccarides, which is not observable in imprints resulting from stamp imprinting.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/d2sm00137c | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Memory B cells (MBCs) differentiate into plasma cells (PCs) or germinal centers (GCs) upon antigen recall. How this decision is programmed is not understood. We found that the relative strength between two antagonistic transcription factors, B lymphocyte-induced maturation protein 1 (BLIMP1) and BTB domain and CNC homolog 2 (BACH2), progressively increases in favor of BLIMP1 in antigen-responding B cells through the course of primary responses.
View Article and Find Full Text PDFFabrication of Large-Area Nanostructures Using Cross-Nanoimprint Strategy.
Nanomaterials (Basel)
June 2024
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, China.
Nanostructures with sufficiently large areas are necessary for the development of practical devices. Current efforts to fabricate large-area nanostructures using step-and-repeat nanoimprint lithography, however, result in either wide seams or low efficiency due to ultraviolet light leakage and the overflow of imprint resin. In this study, we propose an efficient method for large-area nanostructure fabrication using step-and-repeat nanoimprint lithography with a composite mold.
View Article and Find Full Text PDFMultiple Human Population Movements and Cultural Dispersal Events Shaped the Landscape of Chinese Paternal Heritage.
Mol Biol Evol
July 2024
Institute of Rare Diseases, West China Hospital of Sichuan University, Sichuan University, Chengdu 610000, China.
Article Synopsis
- Large-scale genomic projects and innovations in ancient DNA have improved our understanding of human evolution, but the genetic connections of ancient Eurasians to modern Chinese paternal lineages remain unclear.
- Researchers created a Y-chromosome genomic database of 15,563 individuals, revealing multiple population movements and expansions during the Neolithic era, significantly impacting the genetic landscape of modern Chinese people.
- The study identifies four major ancient migrations linked to technological advancements: early East Asian millet farmers, rice farmers, Neolithic Siberian hunter-gatherers, and western Eurasian pastoralists, all of which have shaped the genetic diversity seen in contemporary populations.
Template bacteria-free fabrication of surface imprinted polymer-based biosensor for E. coli detection using photolithographic mimics: Hacking bacterial adhesion.
Biosens Bioelectron
October 2024
Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Campus Jülich, 52428, Jülich, Germany; Institute of Biological Information Processing (IBI-3), Research Centre Jülich GmbH, 52425, Jülich, Germany. Electronic address:
As one class of molecular imprinted polymers (MIPs), surface imprinted polymer (SIP)-based biosensors show great potential in direct whole-bacteria detection. Micro-contact imprinting, that involves stamping the template bacteria immobilized on a substrate into a pre-polymerized polymer matrix, is the most straightforward and prominent method to obtain SIP-based biosensors. However, the major drawbacks of the method arise from the requirement for fresh template bacteria and often non-reproducible bacteria distribution on the stamp substrate.
View Article and Find Full Text PDFSimple Isopropanol-Assisted Direct Soft Imprint Lithography for Residue-Free Microstructure Patterning.
ACS Appl Mater Interfaces
May 2024
Guangdong Basic Research Center of Excellence for Energy & Information Polymer Materials, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Wushan Road 381, Guangzhou 510640, P. R. China.
A direct soft imprint lithography was proposed to realize the direct fabrication of residue-free, well-shaped functional patterns through a single step. This imprint method requires only a simply prepared isopropanol-treated polydimethylsiloxane (PDMS) stamp without any additional resists. Residue-free Ag patterns were successfully fabricated on different substrates by directly imprinting the Ag ink with the isopropanol-treated PDMS stamp.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!