The human liver fluke Opisthorchis viverrini (Ov), the primary risk factor for cholangiocarcinoma (CHCA), is a parasite endemic to southeast Asian countries. With no effective treatments for CHCA currently available, early diagnosis and treatment of Ov infection remains the only practical method for the prevention of CHCA. In this study, plasma phosphoproteomes of patients in the non-Ov infection, non-cholangiocarcinoma subject group (non-OVCCA), the asymptomatic Ov infected group (OV), and the CHCA group (CCA), were investigated to identify potential biomarkers for Ov infection and CHCA. The AKT signalling pathway was found to be up-regulated. Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB), an upstream signalling molecule, was selected as a potential biomarker and evaluated using indirect enzyme-linked immunosorbent assay (ELISA). Results demonstrated evidence that levels of PIK3CB in both the OV group and CCA group was statistically different compared to the non-OVCCA group (P < 0.01). However, the levels of PIK3CB between the OV group and the CCA group were found not to be statistically different. Sensitivity and specificity for OV using OD450 cut-off at >1.570 was 76 and 72%, respectively. For CCA, sensitivity and specificity using OD450 cut-off at >1.398 was 68 and 76%, respectively. Application of indirect ELISA detecting plasma PIK3CB will be of great benefit for screening of opisthorchiasis and CHCA.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11010463 | PMC |
| http://dx.doi.org/10.1017/S0031182021001694 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Learning from Other Tumors: Pathways for Progress and Overcoming Challenges in Cholangiocarcinoma.
Cancers (Basel)
January 2025
Experimental Hepatology and Drug Targeting (HEVEPHARM) Group, University of Salamanca, IBSAL, CIBERehd, Campus M. Unamuno s/n, 37007 Salamanca, Spain.
Cholangiocarcinoma (CCA) is a group of complex and heterogeneous tumors originating from the epithelial cells of bile ducts that can occur in intrahepatic, perihilar, or distal localizations [...
View Article and Find Full Text PDFRichness for Tumor-Infiltrating B-Cells in the Oral Cancer Tumor Microenvironment Is a Prognostic Factor in Early-Stage Disease and Improves Outcome in Advanced-Stage Disease.
Cancers (Basel)
January 2025
Department of Otolaryngology/Head and Neck Surgery, Vrije Universiteit, Amsterdam UMC, Boelelaan 1117, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands.
Background/objectives: Most studies on the interaction between the immune system and cancer focus on T-cells, whereas studies on tumor-infiltrating B-lymphocytes (TIL-Bs) are still underrepresented. The aim of this study was to assess the prognostic impact of TIL-Bs in early- and advanced-stage oral cavity squamous cell carcinoma (OCSCC).
Methods: In total, 222 OCSCCs were studied.
Multivariate patterns among multimodal neuroimaging and clinical, cognitive, and daily functioning characteristics in bipolar disorder.
Neuropsychopharmacology
January 2025
Neurocognition and Emotion in Affective Disorders (NEAD) Centre, Psychiatric Centre Copenhagen, Mental Health Services, Capital Region of Denmark, Frederiksberg, Denmark.
Individuals with bipolar disorder (BD) show heterogeneity in clinical, cognitive, and daily functioning characteristics, which challenges accurate diagnostics and optimal treatment. A key goal is to identify brain-based biomarkers that inform patient stratification and serve as treatment targets. The objective of the present study was to apply a data-driven, multivariate approach to quantify the relationship between multimodal imaging features and behavioral phenotypes in BD.
View Article and Find Full Text PDFApplication of MRI-based tumor heterogeneity analysis for identification and pathologic staging of breast phyllodes tumors.
Magn Reson Imaging
January 2025
Background: Huai-he Hospital of Henan University, Kaifeng, China. Electronic address:
Objective: To explore the application value of MRI-based imaging histology and deep learning model in the identification and classification of breast phyllodes tumors.
Methods: Seventy-seven patients diagnosed as breast phyllodes tumors and fibroadenomas by pathological examination were retrospectively analyzed, and traditional radiomics features, subregion radiomics features, and deep learning features were extracted from MRI images, respectively. The features were screened and modeled using variance selection method, statistical test, random forest importance ranking method, Spearman correlation analysis, least absolute shrinkage and selection operator (LASSO).
Fisher-Centric Methodology: Psychosocial Risks in Fishing Sector of Aotearoa NZ.
J Agromedicine
January 2025
Guard Safety, Health and Safety, Nelson, New Zealand.
Objective: Our paper focuses on the psychosocial risks faced by fishers in Aotearoa New Zealand, a sector with limited existing research on this topic. Using a culture-centered approach (CCA), we aimed to develop "voice infrastructure" to capture and present the voices of fishers, addressing the structural inequalities that can often leave fishers (like other marginalized groups) unheard. This paper focuses on the methodology of our pilot project that used a collaborative effort between academics, fishers, government, and non-government agencies, with the goal of understanding and mitigating the psychosocial risks within the commercial fishing industry.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!