Uterine lumen fluid (ULF) is central to successful pregnancy establishment and maintenance, and impacts offspring wellbeing into adulthood. The current dogma is that ULF composition is primarily governed by endometrial glandular epithelial cell secretions and influenced by progesterone. To investigate the hypothesis that ULF is metabolically semi-autonomous, ULF was obtained from cyclic heifers, and aliquots incubated for various durations prior to analysis by untargeted semi-quantitative metabolomic profiling. Metabolite flux was observed in these ULF isolates, supporting the idea that the biochemical makeup of ULF is semi-autonomously dynamic due to enzyme activities. Subsequent integrative analyses of these, and existing, data predict the specific reactions underpinning this phenomenon. These findings enhance our understanding of the mechanisms leading to pregnancy establishment, with implications for improving fertility and pregnancy outcomes in domestic animals as well as women.
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http://dx.doi.org/10.1038/s42003-022-03134-0 | DOI Listing |
Int J Mol Sci
February 2025
Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
In ruminants, the survival and development of the conceptus are heavily dependent on the composition of the uterine lumen fluid (ULF), which is influenced by prostaglandins (PGs). However, the variations in underlying PG-mediated ULF remain unclear. Herein, cycling heifers received an intrauterine infusion of vehicle as a control (CON) or meloxicam (MEL) on days 12-14 of the estrous cycle.
View Article and Find Full Text PDFDiagnostics (Basel)
March 2025
Department of Radiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-617, RJ, Brazil.
To analyze the microscopic anatomy of the human uterine cervix in two-dimensional (2D) and three-dimensional (3D) images obtained by microtomography (microCT). Human uterine cervixes surgically removed for benign gynecologic conditions were immersed in formalin and iodine solution for more than 72 h and images were acquired by microtomography. In total, 10 cervical specimens were evaluated.
View Article and Find Full Text PDFSci Rep
March 2025
Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, No. 251, Yaojiayuan Road, Chaoyang District, Beijing, 100026, China.
The endometrium is a critical component of female reproductive health. Endometritis can significantly affect women's health, leading to complications such as infertility, pregnancy failure, and intrauterine adhesions. Therefore, establishing a reliable and effective model of endometritis is essential for advancing research in female reproductive health.
View Article and Find Full Text PDFGan To Kagaku Ryoho
February 2025
Dept. of Gastroenterological Surgery, Sakai City Medical Center.
A 66-year-old woman presented with discharge of necrotic tissue and bleeding from the vagina during uterine cancer screening. She was diagnosed with lower rectal cancer cT4b(vagina)N3M0, cStage Ⅲc. As the tumor protruded into the lumen from the posterior vaginal wall, preservation of the anterior vaginal wall was challenging.
View Article and Find Full Text PDFDomest Anim Endocrinol
March 2025
Division of Animal Sciences, Southwest Research, Extension, and Education Center, University of Missouri, Mt Vernon, Missouri, USA.
In cattle, the physiological process of switching from cycling to pregnant is complex. Ultimately, that process relies on endometrial luminal epithelial cells and is based on the paracrine context of the uterine lumen. Cells either release luteolytic pulses of prostaglandin F2 alpha to keep the animal cycling or respond to cues released by the elongated conceptus that block prostaglandin F2 alpha pulses to maintain luteal function and pregnancy.
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