Oxygen generating scaffolds regenerate critical size bone defects.

Recent innovations in bone tissue engineering have introduced biomaterials that generate oxygen to substitute vasculature. This strategy provides the immediate oxygen required for tissue viability and graft maturation. Here we demonstrate a novel oxygen-generating tissue scaffold with predictable oxygen release kinetics and modular material properties. These hydrogel scaffolds were reinforced with microparticles comprised of emulsified calcium peroxide (CaO) within polycaprolactone (PCL). The alterations of the assembled materials produced constructs within 5 ± 0.81 kPa to 34 ± 0.9 kPa in mechanical strength. The mass swelling ratios varied between 11% and 25%. Our and results revealed consistent tissue viability, metabolic activity, and osteogenic differentiation over two weeks. The optimized cell culture system remained stable at pH 8-9. The rodent models demonstrated that these scaffolds support a 70 mm bone volume that was comparable to the native bone and yielded over 90% regeneration in critical size cranial defects. Furthermore, the bone remodeling and vascularization results were validated by tartrate-resistant acid phosphatase (TRAP) and vascular endothelial growth factor (VEGF) staining. The promising results of this work are translatable to a repertoire of regenerative medicine applications including advancement and expansion of bone substitutes and disease models.