Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS.

Food Chem

The National Engineering Research Center of Crop Molecular Breeding, MARA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:

Published: August 2022

AI Article Synopsis

  • Soybeans are a significant source of folates, and a study optimized the extraction and detection of these nutrients, analyzing 1074 different soybean accessions.
  • The total folate content varied greatly, from 64.51 to 691.24 μg/100 g of fresh weight, with 60 elite accessions identified that had over 400 μg/100 g.
  • Factors like seed-coat color, seed weight, and origin significantly influenced folate content, with specific accessions from Northeast China showing higher levels of folate.

Article Abstract

Soybean is a rich source of folates. We optimised the extraction and detection of folates from soybean seeds by HPLC-MS/MS and analysed the folate content and composition of 1074 accessions. Total folate content ranged from 64.51 to 691.24 μg/100 g fresh weight, with 10-fold variation, and 60 elite accessions with over 400 μg/100 g of total folate were identified. The most abundant component was 5-CHO-Hfolate, which accounted for an average of 60% of total folate content. Seed-coat colour, seed weight, ecoregion, and accession type significantly affected soybean folate content. Furthermore, 5-CH-Hfolate correlated positively with seed protein (r = 0.24***) and negatively with oil (r = -0.26***). The geographical distribution of folate according to accession origin revealed that accessions from Northeast China contain higher amounts of total folate and 5-CHO-Hfolate. This study provides comprehensive and novel insights into the folate profile of soybean, which will benefit soybean breeding for folate enhancement.

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http://dx.doi.org/10.1016/j.foodchem.2022.132520DOI Listing

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