AI Article Synopsis
- The paper introduces a new quantitative method for assessing how well non-staphylococci (NAS) can inhibit the growth of mastitis-related bacteria in a lab setting.
- It involved testing various concentrations of NAS on agar and showed significant variability in growth inhibition among NAS isolates, which wasn't captured by traditional semiquantitative methods.
- When applying this method to a larger group of NAS isolates, it was found that certain NAS from teat apices were more effective at lower concentrations in inhibiting both methicillin-sensitive and methicillin-resistant staphylococci.
Article Abstract
In this paper, we describe a new quantitative method to evaluate and quantify in vitro growth inhibition of mastitis-related bacteria. Colony-forming units of ( = 10), ( = 10), and ( = 10) were quantified after their growth on top of layers of trypticase soy agar (TSA) containing six different concentrations (varying from 10 to 10 CFU/mL) of bovine non- staphylococci (NAS), i.e., ( = 3) and ( = 3) isolates. Growth inhibition of the mastitis-related major bacterial pathogens, including , was confirmed by all NAS, an effect that varied highly among NAS isolates and was not evident from the semiquantitative method with which the new method was compared. By subsequent application of the new method on a larger set of 14 bovine NAS isolates, we observed that and NAS originating from teat apices (especially ) required lower concentrations to inhibit both methicillin-sensitive (MSSA) ( = 5) and methicillin-resistant (MRSA) isolates ( = 5) originating from milk. Therefore, the new assay is a promising tool to precisely quantify the intra- and inter-species differences in growth inhibition between NAS.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879122 | PMC |
| http://dx.doi.org/10.3390/pathogens11020264 | DOI Listing |
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