AI Article Synopsis
- Emerging mosquito control technologies often rely on releasing mass quantities of male mosquitoes, but effective global sex-sorting methods are lacking.
- A study discovered a way to target a specific gene involved in sex determination using RNA interference (RNAi), leading to female larval death while not affecting males.
- The research demonstrated that incorporating yeast with the targeted RNAi into larvae diets can produce a high ratio of males to females, potentially enhancing global strategies for controlling mosquito populations via the release of sterile or genetically modified males.
Article Abstract
Although several emerging mosquito control technologies are dependent on mass releases of adult males, methods of sex-sorting that can be implemented globally have not yet been established. RNAi screens led to the discovery of siRNA, which targets gamma-glutamyl transpeptidase (), a gene which is well conserved in multiple species of mosquitoes and located at the sex-determining M locus region in . Silencing the , , and genes resulted in female larval death, with no significant impact on male survival. Generation of yeast strains that permitted affordable expression and oral delivery of shRNA corresponding to mosquito genes facilitated larval target gene silencing and generated significantly increased 5 males:1 female adult ratios in each species. Yeast targeting a conserved sequence in genes was incorporated into a larval mass-rearing diet, permitting the generation of fit adult male and , two species for which labor-intensive manual sex separation had previously been utilized. The results of this study indicate that female-specific yeast-based RNAi larvicides may facilitate global implementation of population-based control strategies that require releases of sterile or genetically modified adult males, and that yeast RNAi strategies can be utilized in various species of mosquitoes that have progressed to different stages of sex chromosome evolution.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879970 | PMC |
| http://dx.doi.org/10.3390/pathogens11020169 | DOI Listing |
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