Successful strategies for the attachment of oligopeptides to mesoporous silica with pores large enough to load biomolecules should utilize the high surface area of pores to provide an accessible, protective environment. A two-step oligopeptide functionalization strategy is examined here using diazirine-based heterobifunctional linkers. Mesoporous silica nanoparticles (MSNPs) with average pore diameter of ~8 nm and surface area of ~730 m/g were synthesized and amine-functionalized. Tetrapeptides Gly-Gly-Gly-Gly (GGGG) and Arg-Ser-Ser-Val (RSSV), and a peptide comprised of four copies of RSSV (4RSSV), were covalently attached via their N-terminus to the amine groups on the particle surface by a heterobifunctional linker, sulfo-succinimidyl 6-(4,4'-azipentanamido)hexanoate (sulfo-NHS-LC-diazirine, or SNLD). SNLD consists of an amine-reactive NHS ester group and UV-activable diazirine group, providing precise control over the sequence of attachment steps. Attachment efficiency of RSSV was measured using fluorescein isothiocyanate (FITC)-tagged RSSV (RSSV-FITC). TGA analysis shows similar efficiency (0.29, 0.31 and 0.26 mol peptide/mol amine, respectively) for 4G, RSSV and 4RSSV, suggesting a generalizable method of peptide conjugation. The technique developed here for the conjugation of peptides to MSNPs provides for their attachment in pores and can be translated to selective peptide-based separation and concentration of therapeutics from aqueous process and waste streams.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880541 | PMC |
| http://dx.doi.org/10.3390/nano12040608 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Drug-Silica-Cellulose Ternary Matrix for the Oral Delivery of Cyclosporine A: and evaluation.
Pharm Dev Technol
January 2025
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Madhavnagar, Manipal - 576104, Karnataka, India.
Purpose: Supersaturated formulations have been widely explored for improving the oral bioavailability of drugs by using mesoporous silica (MS) to generate supersaturation via molecular adsorption; however, this is followed by precipitation. Several precipitation inhibitors (PI) have been explored to prevent precipitation and maintain the drug in solution for a longer period. However, the combined approach of MS and PIs, the impact of MS and Silica, and the loading of high-molecular-weight neutral molecules such as Cyclosporine A (CsA) have not yet been explored.
View Article and Find Full Text PDFPreparation of Robust, Antireflective and Superhydrophobic Hierarchical Coatings on PMMA Substrates via Mechanical Locking and Chemical Bonding.
ACS Appl Mater Interfaces
January 2025
Functional Nanomaterials Laboratory, Center for Micro/Nanomaterials and Technology, and Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Antireflection (AR) coatings with mechanical robustness and superhydrophobic properties have wide potential applications in optical, electronic, and automotive fields. However, the fabrication of large-sized, robust, and multifunctional AR coatings on plastic/polymer substrates has been a challenging problem. In this study, we developed a bottom-up approach to produce mechanically robust, enhanced transmittance, and superhydrophobic coatings on poly(methyl methacrylate) (PMMA) substrate.
View Article and Find Full Text PDFPiperine-loaded mesoporous silica nanoparticles as a promising strategy for targeting Echinococcus granulosus protoscoleces.
Sci Rep
January 2025
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Medical and surgical treatments for cystic echinococcosis (CE) are challenged by various complications. This study evaluates in vitro protoscolicidal activity of piperine-loaded mesoporous silica nanoparticles (PIP-MSNs) against protoscoleces of Echinococcus granulosus. MSNs were prepared by adding tetraethyl orthosilicate to cetyltrimethylammonium bromide and NaOH, and then loaded with PIP.
View Article and Find Full Text PDFRecent Applications of Pillararene-Inspired Water-Soluble Hosts.
Chemistry
January 2025
Shanghai University, Chemistry, 99 Shang-da Road, 200444, Shanghai, CHINA.
Pillararenes and their derivatives have emerged in supramolecular chemistry as unique macrocycles for applications in host-guest chemistry, materials science and biomimetics. Many variations have been conceived and synthesized in recent years and in this review, we relate progress in water-soluble versions: leaning towerarenes, extended-pillararenes, biphenarenes, helicarenes and octopusarenes. These are applied in targeted drug delivery, selective uptake and release of aromatic guests, fabrication of gold/silver and mesoporous silica nanoparticles, cell imaging, pollutant separation, biomedicine (e.
View Article and Find Full Text PDFEvaluation of several strategies for controlling canker plant disease caused by .
Mol Biol Res Commun
January 2025
Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan.
Article Synopsis
- The text discusses a gram-negative bacterium that affects various plants, highlighting the challenges in controlling its rapid spread.
- Several antimicrobial strategies are available, such as chemical agents, biological control, and innovative approaches using secondary metabolites and nanoparticles, with chemical control being the most common yet environmentally concerning.
- Combining treatments, like bacteriophages and antimicrobial peptides with secondary metabolites, could enhance effectiveness, though cost and toxicity concerns exist, suggesting low-concentration approaches may be the most beneficial.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!