Establishment of In Vitro Brain Models for AON Delivery. | LitMetric

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Establishment of In Vitro Brain Models for AON Delivery. | LitMetric

Establishment of In Vitro Brain Models for AON Delivery.

Elena Daoutsali Ronald A M Buijsen

Methods Mol Biol

Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands.

Published: March 2022

Progress in stem cell biology has made it possible to generate human-induced pluripotent stem cells (hiPSC) that can be differentiated into complex, three-dimensional structures, where the cells are spatially organized. To study brain development, Lancaster and colleagues developed an hiPSC-derived three-dimensional organoid culture system, termed cerebral organoids, that develop various discrete, although interdependent, brain regions. Here we describe in detail the generation of cerebral organoids using a modified version of the culture protocol.

Download full-text PDF	Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9703237	PMC
http://dx.doi.org/10.1007/978-1-0716-2010-6_17	DOI Listing

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