Since the inaugural issue of , many changes within publishing have occurred, impacting when, where, and how researchers conduct literature searches for non-animal alternatives. Such changes include increased rate of growth in scientific publications, greater number of databases and online resources available to search, opportunities for open and almost immediate dissemination of research outputs such as preprints and method protocols, and the development of reporting guidelines for animal research. Here we offer a librarian's perspective on these changes and advice on how to manage them to enable robust and diverse alternatives to be implemented in future research.
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Worldwide, many coastal freshwater ecosystems suffer from seawater intrusion. In addition to this stressor, it is likely that the biota inhabiting these ecosystems will also need to deal with climate change-related temperature fluctuations. The resilience of populations to long-term exposure to these stressors will depend on their genetic diversity, a key for their adaptation to changing environments.
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