Monoterpene Indole Alkaloids from the Aerial Parts of Induce Cytotoxicity and Apoptosis in Human Adenocarcinoma Cells.

Chromatographic investigation of the aerial parts of the (Apocynaceae) resulted in the isolation of two new monoterpene indole alkaloids, 6--antirhine--methyl () and razyamide (), along with six known compounds, eburenine (), -rhazyaminine (), rhazizine (), 20--sitsirikine (), antirhine (), and 16--stemmadenine--oxide (). The chemical structures were established by various spectroscopic experiments. Compounds - exhibited cytotoxic effects against three cancer cells with IC values ranging between 5.1 ± 0.10 and 93.2 ± 9.73 µM against MCF-7; 5.1 ± 0.28 and 290.2 ± 7.50 µM against HepG2, and 3.1 ± 0.17 and 55.7 ± 4.29 µM against HeLa cells. Compound showed the most potent cytotoxic effect against all cancer cell lines (MCF-7, HepG2 and HeLa with IC values = 5.1 ± 0.10, 5.1 ± 0.28, and 3.1 ± 0.17 µM, respectively). Furthermore, compound revealed a significant increase in the apoptotic cell population of MCF-7, HepG2, and HeLa cells, with 31.4 ± 0.2%, 29.2 ± 0.5%, and 34.9 ± 0.6%, respectively. Compound decreased the percentage of the phagocytic pathway on HepG2 cells by 15.0 ± 0.1%. These findings can explain the antiproliferative effect of compound .