A credible risk analysis of maize pollen containing Cry1Ab toxin must include the assessment of () pollen production and its Cry1 toxin content; () distribution of the pollen grains in the surroundings; () pollen-catching capacity of the weeds on field edges; () the lifestyle of protected lepidopteran larvae living on weeds; () Cry1 toxin sensitivity of non-target caterpillars; and () Cry1 toxin resistance of individual non-target populations. The concentration range of 5-4300 ng Cry1Ab toxin/g dry pollen determined in MON 810 pollen batches is too diverse for handling it as a single set in any mathematical modeling. Within the work carried out mainly with the DK-440 BTY cultivar, the seed samples officially received from the variety owner produced significantly different (250-470 vs. 5-15 ng/g) Cry1Ab toxin concentrations in the pollen. L1-L3 larvae were nearly six times more sensitive for Dipel than . Feeding on the back side and in a leaf nest, may be subject to lower pollen exposures. larvae may actively attempt to avoid patches with high pollen contamination. Cry1Ab toxin resistance also partially emerged in populations reared in the Pannonian Biogeographical Region (Hungary).
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http://dx.doi.org/10.3390/insects13020206 | DOI Listing |
J Econ Entomol
January 2025
Department of Entomology and Plant Pathology, North Carolina State University, the Vernon G. James Research and Extension Center, Plymouth, NC, USA.
Transgenic corn (Zea mays L.) expressing insecticidal toxins from Bacillus thuringiensis (Bt) helps to control or suppress injury from a range of target insect pests. This study summarizes the yield benefits of Bt corn from field trials in Georgia, North Carolina, and South Carolina evaluating Bt and non-Bt corn hybrids from 2009 to 2023.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Developing simple and efficient multi-gene expression systems is crucial for multi-trait improvement or bioproduction in transgenic plants. In previous research, an -based bicistronic system from the nonpathogenic fungus efficiently expressed multiple enzyme proteins in yeast and maize, and the heterologous enzymes successfully performed their catalytic activity to reconstruct the biosynthetic pathway in the host organism. Unlike enzyme proteins, some heterologous functional proteins (such as insecticidal proteins) are dose-dependent and they need to express sufficient levels to perform their biological functions.
View Article and Find Full Text PDFMikrochim Acta
December 2024
Key Laboratory of Agricultural Genetically Modified Organisms Traceability of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
A multi-chromatic and multi-component lateral flow immunoassay (MCMC-LFIA) was developed for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins in genetically modified (GM) crops. Captured antibodies specific to these exogenous proteins were separately immobilized on a nitrocellulose membrane as test zones. Multi-colored microspheres, used as visible multi-probes, were conjugated with corresponding antibodies and sprayed on the conjugate pad.
View Article and Find Full Text PDFInsects
November 2024
Key Laboratory on Biodiversity and Biosafety, Nanjing Institute of Environmental Sciences, MEE, Nanjing 210042, China.
Transgenic poplars have been used to control quarantine pests worldwide, such as the fall webworm (, FW). However, the studies on the resistance mechanism of FW to Cry toxins are limited. This study obtained an FW strain with 45-fold resistance to Cry1Ab toxin by continuous screening in the laboratory.
View Article and Find Full Text PDFInt J Biol Macromol
November 2024
State Key Laboratory of Rice Biology, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310000, Zhejiang Province, China; Hangzhou Ruifeng Biosciences Co., Ltd., 1500 Wenyi Road, Building 1, Room103, Hangzhou 310000, Zhejiang Province, China. Electronic address:
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