AI Article Synopsis
- The study aims to improve a molecular imaging agent, NACA, for better detection of tumor necrosis, which can indicate the effectiveness of cancer treatments.
- The researchers modified the NACA to enhance its binding to albumin, increasing its blood circulation time and tested its ability to identify dead vs. living cancer cells using advanced imaging techniques in mouse models.
- Results showed that the new NACA had significantly higher binding to dead cells, improved imaging of necrosis in treated tumors, and allowed for better assessment of treatment efficacy compared to standard imaging methods.
Article Abstract
Purpose: To assess our improved NACA for the detection of tumor necrosis.
Methods: We increased the blood circulation time of our NACA by adding an albumin-binding domain to the molecular structure. We tested the necrosis avidity on dead or alive cultured cells and performed SPECT and fluorescence imaging of both spontaneous and treatment-induced necrosis in murine breast cancer models. We simultaneously recorded [F]FDG-PET and bioluminescence images for complementary detection of tumor viability.
Results: We generated two albumin-binding IRDye800CW derivatives which were labeled with indium-111 with high radiochemical purity. Surprisingly, both albumin-binding NACAs had >10x higher in vitro binding towards dead cells. We selected [In] for in vivo experiments which showed higher dead cell binding in vitro and in vivo stability. The doxorubicin-treated tumors showed increased [In]-uptake (1.74 ± 0.08%ID/g after saline treatment, 2.25 ± 0.16%ID/g after doxorubicin treatment, = 0.044) and decreased [F]FDG-uptake (3.02 ± 0.51%ID/g after saline treatment, 1.79 ± 0.11%ID/g after doxorubicin treatment, = 0.040), indicating therapy efficacy. Moreover, we detected increased [In]uptake and tumor necrosis in more rapidly growing EMT6 tumors.
Conclusions: Our albumin-binding NACA based on IRDye800CW facilitates tumor-necrosis imaging for assessment of therapy efficacy and aggressiveness in solid tumors using both fluorescence and SPECT imaging.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870237 | PMC |
| http://dx.doi.org/10.3390/cancers14040861 | DOI Listing |
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