AI Article Synopsis
- Macrophages are diverse immune cells that play roles in both maintaining body functions and responding to infections, existing in two main forms: pro-inflammatory (M1) and anti-inflammatory (M2).
- The M1 and M2 phenotypes can be mimicked in lab settings using certain substances that activate toll-like receptors and specific cytokines like IFNγ and IL-4.
- This study focused on macrophages derived from human-induced pluripotent stem cells (iPSCs), which were polarized to M1 or M2 types, and used advanced proteomics to analyze their protein expressions and secretions, shedding light on their varied functions.
Article Abstract
Macrophages (M) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M can exist in two extreme phenotypes: pro-inflammatory (M1) M and anti-inflammatory (M2) M. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived M have gained major attention, as they are functionally similar to human monocyte-derived M and are receptive to genome editing. In this study, we polarised iPSC-derived M to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised M.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869710 | PMC |
| http://dx.doi.org/10.3390/biomedicines10020239 | DOI Listing |
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