Objective: The neonatal mouse retina is a well-characterized experimental model for investigating factors impacting retinal angiogenesis and inner blood-retinal barrier (BRB) integrity. Retinoic acid (RA) is an essential signaling molecule. RA is needed for vasculogenic development in embryos and endothelial barrier integrity in zebrafish retina and adult mouse brain; however, the function of this signaling molecule in developing mammalian retinal vasculature remains unknown. This study aims to investigate the role of RA signaling in angiogenesis and inner BRB integrity in mouse neonatal retina.
Methods: RA distribution in the developing neurovascular retina was assessed in mice carrying an RA-responsive transgene. RA function in retinal angiogenesis was determined by treating C57BL/6 neonatal pups with a pharmacological inhibitor of RA signaling BMS493 or control vehicle. BRB integrity assessed by monitoring leakage of injected tracer into extravascular retinal tissue.
Results: RA signaling activity is present in peripheral astrocytes in domains corresponding to RA activity of the underlying neural retina. RA inhibition impaired retinal angiogenesis and reduced endothelial cell proliferation. RA inhibition also compromised BRB integrity. Vascular leakage was not associated with altered expression of CLDN5, PLVAP, LEF1, or VEcad.
Conclusions: RA signaling is needed for angiogenesis and integrity of the BRB in the neonatal mouse retina.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/micc.12752 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Interplay between Netrin-1 and Norrin controls arteriovenous zonation of blood-retina barrier integrity.
Proc Natl Acad Sci U S A
December 2024
Paris Cardiovascular Research Center, Université Paris Cité, Inserm U970, Paris F-75015, France.
The integrity of the blood-retina barrier (BRB) is crucial for phototransduction and vision, by tightly restricting transport of molecules between the blood and surrounding neuronal cells. Breakdown of the BRB leads to the development of retinal diseases. Here, we show that Netrin-1/Unc5b and Norrin/Lrp5 signaling establish a zonated endothelial cell gene expression program that controls BRB integrity.
View Article and Find Full Text PDFThe integrity of the blood-retinal barrier (BRB) has been largely unexplored in glaucoma. We reveal that elevated intraocular pressure (IOP) partially compromises the BRB in two human-relevant inherited mouse models of glaucoma (DBA/2J and Lmx1bV265D). Experimentally increasing IOP in mouse eyes further confirms this.
View Article and Find Full Text PDFPathological R-loops in bacteria from engineered expression of endogenous antisense RNAs whose synthesis is ordinarily terminated by Rho.
Nucleic Acids Res
November 2024
Indian Institute of Science Education and Research Mohali, Sector 81, SAS Nagar 140306, Punjab, India.
In many bacteria, the essential factors Rho and NusG mediate termination of synthesis of nascent transcripts (including antisense RNAs) that are not being simultaneously translated. It has been proposed that in Rho's absence toxic RNA-DNA hybrids (R-loops) may be generated from nascent untranslated transcripts, and genome-wide mapping studies in Escherichia coli have identified putative loci of R-loop formation from more than 100 endogenous antisense transcripts that are synthesized only in a Rho-deficient strain. Here we provide evidence that engineered expression in wild-type E.
View Article and Find Full Text PDFRemote ischemic conditioning slows blood-retinal barrier damage in type 1 diabetic rats.
Brain Res
January 2025
Department of Ophthalmology, Xuanwu Hospital of Capital Medical University, Beijing, China. Electronic address:
Diabetic retinopathy (DR) is one of the major complications of diabetes and can cause severe visual impairment. Blood-retina barrier (BRB) destruction resulted from chronic hyperglycemia underlines its major pathological process. However, current treatments have limited efficacy and may even cause serious complications.
View Article and Find Full Text PDF2D/1D VSe/MWCNT hybrid-based electrochemical sensor for carbendazim quantification of environmental, food, and biological samples.
Mikrochim Acta
August 2024
Centre for Nano and Material Sciences, Jain (Deemed-to-Be University), Jain Global Campus, Kanakapura Road, Bangalore, 562112, Karnataka, India.
Article Synopsis
- * Optimal sensor performance was achieved at pH 8.0, exhibiting a detection limit of 9.80 nM and a wide linear detection range of 0.1 to 10.0 µM, indicating excellent capabilities for real-time quantification of CRB in water, food, and biological samples with recovery rates between 98.6% and 101.5%.
- * The research highlights the mechanism behind the sensor's operation, revealing that charge transfer
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!