is an exceptionally transparent model to analyze calcium (Ca) signals, but available protocols for neuronal Ca2 imaging may not be suitable for studying glial cells. Here, we present a detailed protocol for glial Ca imaging in following three different approaches including chemical, mechanical, and optogenetic stimulation. We also provide the details for imaging analysis using Image-J. For complete details on the use and execution of this protocol, please refer to Duan et al. (2020).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844900 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101169 | DOI Listing |
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