Identification of new ETV6 modulators through a high-throughput functional screening.

transcriptional activity is critical for proper blood cell development in the bone marrow. Despite the accumulating body of evidence linking malfunction to hematological malignancies, its regulatory network remains unclear. To uncover genes that modulate repressive transcriptional activity, we performed a specifically designed, unbiased genome-wide shRNA screen in pre-B acute lymphoblastic leukemia cells. Following an extensive validation process, we identified 13 shRNAs inducing overexpression of transcriptional target genes. We showed that the silencing of , , , and led to an abrogation of repressive activity. We identified critical modulators of the function which could participate in cellular transformation through the transcriptional network.