Toxicological effect of sumithion pesticide on the hematological parameters and its recovery pattern using probiotic in .

The experiment was conducted to clarify sumithion induced hematoxicity in silver barb () through in vivo exposures (25 % and 50 % of LC of sumithion) and subsequent recovery patterns using normal and probiotic treated feed were also assessed. Three treatments each incorporating three replications were used in the experiment for different days (1, 7, 14, 21, and 28). Treatment T was control (0 mg/L), and two concentrations, such as 2.61 mg/L (25 % of 96 h LC), 5.21 mg/L (50 % of 96 h LC) were used as Treatment T and T, respectively. After 28 days of exposure to pesticide half of the fishes of T and T were reared in sumithion free water with normal (TN, TN) and probiotic treated feed (TP, TP). The median lethal concentration (50 %) for 96 h was 10.42 mg/L. In pesticide-treated groups, values of each hematological parameter (blood glucose, red blood cell, hematocrit, and hemoglobin) decreased but prevalence and severity of micronucleus and white blood cells increased significantly (< 0.05) with concentration and time duration. Other blood indices including mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were correspondingly changed in comparison to the control. In the recovery experiment, the silver barb recovered spontaneously, but the recovery rate was significantly higher in probiotic treated groups than normally treated groups in time and duration reliant fashion. In conclusion, persistent sublethal dosages of sumithion caused hematological abnormalities in silver barb. Probiotic supplement can recover the damage but only 28 days of recovery is not enough to recover the total alterations.