AI Article Synopsis
- * A new high-resolution analysis technique improved the determination of jagged end lengths by using methylation at non-CpG cytosine sites, overcoming limitations of previous methods.
- * The research revealed that urinary DNA jagged ends showed a periodic pattern (10-nt) that was more prominent in patients with renal cell carcinoma and decreased with heparin treatment, indicating potential interaction with histones.
Article Abstract
Single-stranded ends of double-stranded DNA (jagged ends) are more abundant in urinary DNA than in plasma DNA. However, the lengths of jagged ends in urinary DNA remained undetermined, as a previous method used for urinary DNA jagged end sequencing analysis (Jag-seq) relied on unmethylation at CpG sites, limiting the resolution. Here, we performed high-resolution Jag-seq analysis using methylation at non-CpG cytosine sites, allowing determination of exact length of jagged ends. The urinary DNA bore longer jagged ends (~26-nt) than plasma DNA (~17-nt). The jagged end length distribution displayed 10-nt periodicities in urinary DNA, which were much less observable in plasma DNA. Amplitude of the 10-nt periodicities increased in patients with renal cell carcinoma. Heparin treatment of urine diminished the 10-nt periodicities. The urinary DNA jagged ends often extended into nucleosomal cores, suggesting potential interactions with histones. This study has thus advanced our knowledge of jagged ends in urine DNA.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866458 | PMC |
| http://dx.doi.org/10.1038/s41525-022-00285-1 | DOI Listing |
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