AI Article Synopsis
- Methionine is a crucial amino acid involved in various biological functions like protein synthesis and cancer metabolism, but its visualization in cells is challenging.
- Researchers used stimulated Raman scattering (SRS) imaging to track deuterated methionine (d-Met) in live HeLa cells and compared it with a previous method using homopropargylglycine (Hpg).
- They found that d-Met provided a stronger SRS signal, indicating better cellular uptake, which may facilitate the study of metabolic processes at a detailed subcellular level using deuterated biomolecules.
Article Abstract
The small biomolecule methionine (Met) is a fundamental amino acid required for a vast range of biological processes such as protein synthesis, cancer metabolism, and epigenetics. However, it is still difficult to visualize the subcellular distribution of small biomolecules including Met in a minimally invasive manner. Here, we demonstrate stimulated Raman scattering (SRS) imaging of cellular uptake of deuterated methionine (d-Met) in live HeLa cells by way of comparison to the previously used alkyne-labeled Met analogue─homopropargylglycine (Hpg). We show that the solutions of d-Met and Hpg have similar SRS signal intensities. Furthermore, by careful image analysis with background subtraction, we succeed in the SRS imaging of cellular uptake of d-Met with a much greater signal intensity than Hpg, possibly reflecting the increased and minimally invasive uptake kinetics of d-Met compared with Hpg. We anticipate that d-Met and other deuterated biomolecules will be useful for investigating metabolic processes with subcellular resolution.
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| http://dx.doi.org/10.1021/acs.jpcb.1c08343 | DOI Listing |
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