Phytochemicals Analysis and Some Bioactive Properties of Salisb. (EMS); Antibacterial, Antiradical and Anti-lipid Peroxidation.

In the present study, the phenolic content by using LC MS/MS method, anticholinergic, antioxidant (metal reduction, radical and removal of lipid peroxidation), and antibacterial activities of Salisb. (EMS) extract were determined. The amount of vanillic acid, fumaric acid, catechin hydrate, quercetin, and phloridzin dihydrate were found higher than other compounds. The ethanol extract of the EMS showed an inhibition effect on the Acetylcholinesterase (AChE) enzyme with IC value of 0.124 ± 0.008 mg mL. Also, this extract of the EMS indicated radical (DPPH and ABTS) scavenging activity (about 20%) and the reducing capacity for Cu(II) and Fe(III) close to trolox, and inhibited the oxidation of linoleic acid with 40.5% at 20 mL. Antibacterial activity of the extracts was investigated against , and Typhimurium using agar disc diffusion and minimum inhibitory concentration (MIC) methods. The EMS extract was found to be effective when used at least 312 mg mL concentration on the pathogenic microorganisms. Consequently, it has an important non-synthetic natural content that can be used in the treatment of many diseases due to its many bioactivities such as anticholinergic, antioxidant (radical removal, lipid peroxidation prevention, etc.) and antibacterial.