AI Article Synopsis
- Researchers engineered 25 new interferons based on human beta-interferon by altering its gene structure and expressing them in E. coli.
- Out of the 25 variants, 23 showed both antiviral (AV) and antiproliferative (AP) activities, some significantly exceeding the effects of natural beta-interferon.
- Changes to specific beta-interferon amino acids greatly enhanced AP activity, while most modified interferons still interacted with a specific neutralizing antibody, indicating they retain some characteristics of the original.
Article Abstract
A series of novel, modified interferons based on the structure of human beta-interferon have been expressed in Escherichia coli. Modified interferon genes were constructed from sequences derived from the natural beta-interferon gene, a synthetic beta-interferon gene, or a specific combination of the two. A total of 23 out of the 25 novel interferons exhibited antiviral (AV) and antiproliferative (AP) activity which varied from 3 to 230% and 8 to 490% of the values for beta-interferon, respectively. None of the novel interferons had only AV or AP activity, although one had a much reduced ratio of AV/AP activity compared with beta-interferon. Substitution of beta-interferon amino acids 2-7 or 28-46 resulted in interferons with significantly increased AP activity on Daudi lymphoblastoid cells (four- to fivefold). All the novel interferons except two with modifications in the 82-105 region reacted with a neutralizing beta-interferon monoclonal antibody.
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| http://dx.doi.org/10.1089/dna.1986.5.137 | DOI Listing |
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