Determination of phosphorus compounds in plant tissues: from colourimetry to advanced instrumental analytical chemistry.

Although the spectrum of effective methods and techniques that allow determination of inorganic or total phosphorus is impressive, more precise analysis of these substances in plant tissues is not a routine or trivial task. The complexity of chemical composition of plant tissues treated as the analytical matrices is thought to be the main cause why there is no one answer, how appropriate phosphorus compounds may be determined qualitatively and quantitatively. Even if more advanced spectrophotometric measurements and classical variants of absorption (FAAS) or emission (ICP-AES/ ICP-OES) spectrometry techniques are used, it is necessary at first to isolate various forms of phosphorus from the matrix, and then to mineralize them prior the determination. Significant progress in such a kind of analytical efforts was brought by implementation of combined methods e.g. ETV-ICP-AES or HR-ETAAS, does allow the isolation of the phosphorus analyte and its detection during a kind of "one step" analytical procedure, directly in plant tissues. Similar benefits, regarding sensitivity of determinations, are obtained when XRF, SIMS or nanoSIMS-more expensive techniques of imaging the presence of phosphorus in biological matrices have been used. Nowadays, obviously being aware of higher limit of detection, nuclear magnetic resonance spectroscopy, especially the P NMR technique, is thought to be the most universal analytical tool allowing to determine various chemical forms of plant phosphorus qualitatively and quantitatively, at the same time. Although P NMR provides valuable information about the phosphorus profile of plants, it should be emphasized that each analytical issue related to the determination of phosphorus compounds in plant tissues and organs, requires an individual approach to defined problem.