AI Article Synopsis
- Sanitary monitoring for SARS-CoV-2 remains essential as social events resume and economies reboot, prompting the evaluation of a rapid saliva test.
- A study involving 443 outpatients found the RT-LAMP saliva test showed 85.9% sensitivity and 99.5% specificity compared to the standard RT-qPCR test, effectively identifying infected individuals regardless of symptoms.
- This innovative self-collected saliva test could streamline virus detection and reporting, making it a valuable tool for ensuring safety at social and professional gatherings in the context of economic recovery.
Article Abstract
In the context of social events reopening and economic relaunch, sanitary surveillance of SARS-CoV-2 infection is still required. Here, we evaluated the diagnostic performances of a rapid, extraction-free and connected reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay on saliva. Nasopharyngeal (NP) swabs and saliva from 443 outpatients were collected simultaneously and tested by reverse-transcription quantitative PCR (RT-qPCR) as reference standard test. Seventy-one individuals (16.0%) were positive by NP and/or salivary RT-qPCR. Sensitivity and specificity of salivary RT-LAMP were 85.9% (95%CI 77.8-94.0%) and 99.5% (98.7-100%), respectively. Performances were similar for symptomatic and asymptomatic participants. Moreover, SARS-CoV-2 genetic variants were analyzed and no dominant mutation in RT-LAMP primer region was observed during the period of the study. We demonstrated that this RT-LAMP test on self-collected saliva is reliable for SARS-CoV-2 detection. This simple connected test with optional automatic results transfer to health authorities is unique and opens the way to secure professional and social events in actual context of economics restart.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857239 | PMC |
| http://dx.doi.org/10.1038/s41598-022-04826-7 | DOI Listing |
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