AI Article Synopsis

  • The stereocilia rootlet helps keep hair cells in the ear stable and protects them from damage so we can hear properly.
  • A protein called ANKRD24 forms a special ring at the rootlet, working closely with another protein, TRIOBP-5, to keep the structure strong and in the right place.
  • When ANKRD24 is missing, it can lead to hearing loss and can make it harder for the ears to recover from loud noises.

Article Abstract

The stereocilia rootlet is a key structure in vertebrate hair cells, anchoring stereocilia firmly into the cell's cuticular plate and protecting them from overstimulation. Using superresolution microscopy, we show that the ankyrin-repeat protein ANKRD24 concentrates at the stereocilia insertion point, forming a ring at the junction between the lower and upper rootlets. Annular ANKRD24 continues into the lower rootlet, where it surrounds and binds TRIOBP-5, which itself bundles rootlet F-actin. TRIOBP-5 is mislocalized in Ankrd24KO/KO hair cells, and ANKRD24 no longer localizes with rootlets in mice lacking TRIOBP-5; exogenous DsRed-TRIOBP-5 restores endogenous ANKRD24 to rootlets in these mice. Ankrd24KO/KO mice show progressive hearing loss and diminished recovery of auditory function after noise damage, as well as increased susceptibility to overstimulation of the hair bundle. We propose that ANKRD24 bridges the apical plasma membrane with the lower rootlet, maintaining a normal distribution of TRIOBP-5. Together with TRIOBP-5, ANKRD24 organizes rootlets to enable hearing with long-term resilience.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859912PMC
http://dx.doi.org/10.1083/jcb.202109134DOI Listing

Publication Analysis

Top Keywords

ankrd24 organizes
8
stereocilia insertion
8
hair cells
8
lower rootlet
8
rootlets mice
8
ankrd24
7
triobp-5
5
organizes triobp
4
triobp reinforce
4
stereocilia
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!